Bisulfite Conversion & Methylation Readout

How bisulfite converts unmodified cytosine to uracil/thymine while 5-methylcytosine and 5-hydroxymethylcytosine are both largely protected, making standard bisulfite readout a combined protected-cytosine signal.

References

• Frommer, M., McDonald, L.E., Millar, D.S., et al. "A Genomic Sequencing Protocol." Proceedings of the National Academy of Sciences 89, no. 5 (1992): 1827–1831.