Chain-Termination Sequencing

How mixing in nucleotides that stop DNA synthesis the moment they're incorporated produces fragments of every possible length, each one revealing the base at its final position.

References

• Sanger, F., Nicklen, S., Coulson, A.R. "DNA Sequencing with Chain-Terminating Inhibitors." Proceedings of the National Academy of Sciences 74, no. 12 (1977): 5463–5467.