Predicting Off-Target Risk From Sequence Similarity Alone

Why searching a whole genome for sequences that closely, but imperfectly, resemble an intended guide sequence predicts where unintended editing might occur, before any experiment is ever run.

References

• Mali, P., Yang, L., Esvelt, K.M., et al. "RNA-Guided Human Genome Engineering." Science 339, no. 6121 (2013): 823–826.