CRISPR Guide RNA Design

Computationally selects the best candidate guide RNA sequence for a gene-editing experiment, predicting which candidates will cut efficiently at the intended target while avoiding likely off-target sites elsewhere in the genome.

References

• Jinek, M., Chylinski, K., Fonfara, I., et al. "Programmable RNA-Guided DNA Endonuclease." Science 337, no. 6096 (2012): 816–821.