Single-Particle Cryo-EM

Determines 3D molecular structure by computationally combining images of many individual flash-frozen molecules captured with an electron beam.

References

• Dubochet, J., Adrian, M., Chang, J.J., Homo, J.C., Lepault, J., McDowall, A.W., Schultz, P. "Cryo-Electron Microscopy of Vitrified Specimens." Quarterly Reviews of Biophysics 21, no. 2 (1988): 129–228.