Differential Cell Wall Staining

How differences in bacterial envelope architecture and solvent-mediated decolorization, not cell-wall thickness alone, cause differential retention of the crystal-violet-iodine complex.

References

• Gram, C. "Über die isolierte Färbung der Schizomyceten in Schnitt- und Trockenpräparaten." Fortschritte der Medicin 2 (1884): 185–189.