Heavy-Metal Tagging as a High-Dimensional Single-Cell Readout

Why labeling antibodies with distinct heavy-metal isotopes, rather than fluorescent dyes, lets far more markers be measured on the same single cell without spectral overlap.

References

• Bodenmiller, B., Zunder, E.R., Finck, R., et al. "Multiplexed Mass Cytometry Profiling." Nature Biotechnology 30, no. 9 (2012): 858–867.