Enzyme Kinetics Assay

Measures reaction rates across controlled substrate concentrations to estimate kinetic parameters such as Vmax, Km, kcat, or kcat/Km under a specified kinetic model and conditions.

References

• Michaelis, L., Menten, M.L. "Die Kinetik der Invertinwirkung." Biochemische Zeitschrift 49 (1913): 333–369.