Fluorescence Polarization Assay

Reads binding directly from how fast a fluorescently labeled molecule tumbles in solution, since binding to something larger slows its tumbling and changes the polarization of light it emits.

References

• Jameson, D.M., Croney, J.C. "Fluorescence Polarization: A Tool for High Throughput Screening." Current Opinion in Chemical Biology 7, no. 1 (2003): 5–9.