Fluorescence Recovery & Molecular Mobility

Why watching how quickly unbleached molecules replace deliberately destroyed ones in a small region converts invisible molecular movement into an easily measured recovery curve.

References

• Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E., Webb, W.W. "Mobility Measurement by Analysis of Fluorescence Photobleaching Recovery Kinetics." Biophysical Journal 16, no. 9 (1976): 1055–1069.