FRAP

Measures how mobile a labeled molecule is by permanently bleaching the fluorophores in one small region and watching how quickly fluorescence returns as unbleached molecules move in.

References

• Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E., Webb, W.W. "Mobility Measurement by Analysis of Fluorescence Photobleaching Recovery Kinetics." Biophysical Journal 16, no. 9 (1976): 1055–1069.