Golden Gate Assembly

Joins multiple DNA fragments in a defined order in a single reaction, using enzymes that cut outside their own recognition sequence so no scar remains at the final junctions.

References

• Engler, C., Kandzia, R., Marillonnet, S. "A One Pot, One Step, Precision Cloning Method with High-Throughput Capability." PLOS One 3, no. 11 (2008): e3647.