Hydrophobic Interaction Chromatography

Separates proteins by their surface hydrophobicity, binding them to a mildly hydrophobic resin in high salt and releasing them gently as the salt concentration drops.

References

• Er-el, Z., Zaidenzaig, Y., Shaltiel, S. "Hydrocarbon-Coated Sepharoses. Use in the Purification of Glycogen Phosphorylase." Biochemical and Biophysical Research Communications 49, no. 2 (1972): 383–390.