Self-Generated pH Gradients and Isoelectric Elution

Why letting a resin and buffer interact to build their own smooth, continuous pH gradient inside the column resolves proteins by isoelectric point more finely than an externally mixed gradient typically can.

References

• Sluyterman, L.A., Wijdenes, J. "Isoelectric Focusing." Journal of Chromatography 206 (1981): 429–440.