Overlap-Based Seamless DNA Assembly

How designing matching overlapping ends onto DNA fragments lets enzymes join them correctly and in order, without relying on restriction sites.

References

• Höss, M., Robins, H., Heyer, W.D., Kolodner, R.D. "Homologous Recombination and Illegitimate Recombination." Current Opinion in Genetics & Development 2, no. 6 (1992): 951–957.