Absolute Quantification by Counting Positive Partitions

Why the fraction of positive partitions, interpreted with Poisson occupancy statistics and partition volume, yields an absolute target concentration.

References

• Sykes, P.F., Neoh, S.H., Brisco, M.J., et al. "Quantitation of Targets for PCR." BioTechniques 13, no. 3 (1992): 444–449.