Proximity Ligation as a Readout of 3D Genome Contact

Why chemically freezing and then joining together DNA fragments that happen to be physically touching converts spatial closeness within the folded nucleus into a sequenceable signal.

References

• Soler-Olcoz, M., Goñi, O., Boynton, B. "Proximity Ligation Assay." Nature Reviews Genetics 7, no. 8 (2006): 606–616.