Quantitative PCR (qPCR)

Quantifies a specific DNA or cDNA target by monitoring fluorescence during PCR amplification; quantifying an RNA target requires a preceding reverse-transcription step (RT-qPCR).

References

• Heid, C.A., Stevens, J., Livak, K.J., Williams, P.M. "Real Time Quantitative PCR." Genome Research 6, no. 10 (1996): 986–994.