Search-and-Replace Editing via a Guide-Encoded Template

How a guide RNA that carries both a target address and the desired new sequence lets an enzyme directly write a precise edit without ever cutting both DNA strands.

References

• Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A., Liu, D.R. "Search-and-Replace Genome Editing without Double-Strand Breaks or Donor DNA." Nature 576, no. 7785 (2019): 149–157.