Ribosome Profiling

Maps ribosome-protected mRNA footprints to identify translated regions and ribosome occupancy; translation efficiency is inferred by normalizing footprint signal to matched transcript abundance.

References

• Ingolia, N.T., Ghaemmaghami, S., Newman, J.R.S., Weissman, J.S. "Genome-Wide Analysis in Vivo of Translation with Nucleotide Resolution Using Ribosome Profiling." Science 324, no. 5924 (2009): 218–223.