Sanger Sequencing

Reads a DNA fragment's exact sequence by synthesizing many copies that randomly terminate at every position, then reading off fragment lengths in order.

References

• Sanger, F., Nicklen, S., Coulson, A.R. "DNA Sequencing with Chain-Terminating Inhibitors." Proceedings of the National Academy of Sciences 74, no. 12 (1977): 5463–5467.