Selecting an Effective Guide RNA Before Running an Experiment

An effective, specific guide RNA sequence for a gene-editing experiment needs to be selected computationally, before synthesizing any reagent or running an actual editing experiment.

References

• Mali, P., Yang, L., Esvelt, K.M., et al. "RNA-Guided Human Genome Engineering." Science 339, no. 6121 (2013): 823–826.