Selective Plane Illumination

How illuminating only a single thin plane of a sample from the side, rather than the whole volume, achieves optical sectioning without needing to reject unwanted light afterward.

References

• Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J., Stelzer, E.H.K. "Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy." Science 305, no. 5686 (2004): 1007–1009.