Single-Cell Cytokine Secretion Detection

How capturing a secreted signal right where a cell releases it preserves single-cell resolution that pooling the sample would destroy.

References

• Czerkinsky, C.C., Nilsson, L.A., Nygren, H., Ouchterlony, O., Tarkowski, A. "A Solid-Phase Enzyme-Linked Immunospot (ELISPOT) Assay for Enumeration of Specific Antibody-Secreting Cells." Journal of Immunological Methods 65, nos. 1–2 (1983): 109–121.