Single-Molecule Localization & Super-Resolution

How separating overlapping fluorophores in time, rather than trying to resolve them in space, lets each one's position be pinpointed far more precisely than diffraction alone allows.

References

• Rust, M.J., Bates, M., Zhuang, X. "Sub-Diffraction-Limit Imaging." Nature Methods 3, no. 10 (2006): 793–795.